LC-MS/MS-based targeted proteomics quantitatively detects the interaction between p53 and MDM2 in breast cancer

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• 作者：Wen Zhang ; Ting Zhong ; Yun Chen ; ^{ychen@njmu.edu.cn}
• 关键词：p53 ; MDM2 ; Protein-protein interaction ; Quantitative targeted proteomics ; Breast cancer
• 刊名：Journal of Proteomics
• 出版年：2017
• 出版时间：30 January 2017
• 年：2017
• 卷：152
• 期：Complete
• 页码：172-180
• 全文大小：1327 K
• 卷排序：152

文摘

Quantitatively detect p53-MDM2 interaction using LC-MS/MS-based targeted proteomics The assay circumvents the trade-off between sensitivity and specificity. The assay provides an accurate and dynamic value of protein-protein interaction. Observe differential p53-MDM2 interaction between normal and breast cancer cases