Facile colorimetric assay of alkaline phosphatase activity using Fe(II)-phenanthroline reporter

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• 作者：Qiong Hu^a ; ¹ ; Baojing Zhou^b ; ¹ ; Pengyun Dang^b ; Lianzhi Li^c ; Jinming Kong^a ; ^{j.kong@njust.edu.cn} ; Xueji Zhang^a ; ^d ; ^{xueji@usf.edu}
• 关键词：Alkaline phosphatase ; Colorimetric ; Density functional theory ; Electrochemical ; Bathophenanthroline disulfonate ; Metal-to-ligand charge-transfer
• 刊名：Analytica Chimica Acta
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：950
• 期：Complete
• 页码：170-177
• 全文大小：1699 K
• 卷排序：950

文摘

A Fe(II)-phenanthroline reporter was used for the colorimetric assay of ALP activity. The approach is based on the distinctive MLCT absorption properties of the reporter. It is highly selective and holds great potential in ALP inhibitor screening. It holds considerable simplicity and flexibility with respect to reporter design. It was successfully employed to detect the endogenous ALP level of serum samples.