Microfluidic-based G-quadruplex ligand displacement assay for alkaloid anticancer drug screening

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• 作者：Haihui Shen¹ ; Bo Zhang ; Huiyan Xu ; Yue Sun¹ ; Qiwang Wu ; Hong Shen ; ^{shzju@zju.edu.cn} ; Yingchun Liu ; ^{liuyingch@zju.edu.cn}
• 关键词：G4 ; G-quadruplex ; CD ; circular dichroism ; CL ; chemiluminescence ; MD ; molecular dynamics ; SAU ; sanguinarine ; BER ; berberine ; CHE ; chelerythrine ; MAT ; matrine ; LIG ; ligustrazine ; PMT ; photomultiplier tube ; PME ; Particle mesh Ewald method ; GAFF ; general AMBER force field ; VMD ; visual molecular dynamics ; PDB ; Protein Data Bank
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2017
• 出版时间：5 February 2017
• 年：2017
• 卷：134
• 期：Complete
• 页码：333-339
• 全文大小：2035 K
• 卷排序：134

文摘

Ligand displacement assay based on DNAzyme-catalyzed CL detection was developed for screening anticancer drug via stabilizing promoter DNA. Alkaloid/G4 DNA interaction of end-stacking mode between alkaloid and Pu22 DNA demonstrated the feasibility of the proposed mechanism. Microfluidic-based CL approach showed the advantage of automatic, high throughput with just trace amount of sample and reagent consumption.