Evaluation of ¹⁸F-FDG and ¹⁸F-FLT for monitoring therapeutic responses of colorectal cancer cells to radiotherapy

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• 作者：Hui Wang ; Bo Liu ; Jiahe Tian ; Baixuan Xu ; Jinming Zhang ; Baolin Qu ; Yingmao Chen
• 关键词：18F-Fluorothymidine ; 18F-Fluorodeoxyglucose ; Irradiation ; Positron emission tomography ; Colorectal cancer
• 刊名：European Journal of Radiology
• 出版年：2013
• 出版时间：September, 2013
• 年：2013
• 卷：82
• 期：9
• 页码：e484-e491
• 全文大小：2858 K

文摘

In order to compare the efficacy of ¹⁸F-fluorothymidine (FLT) and ¹⁸F-fluorodeoxyglucose (FDG) for monitoring early responses to irradiation, two human colorectal cancer (CRC) cell lines SW480 and SW620, which were derived from the primary lesions and the metastatic lymph node, underwent X-ray irradiation of 0, 10, or 20 Gy and were examined at 0, 24 and 72 h After irradiation, reduced proliferation of both SW480 and SW620 cells was observed in a dose-dependent manner (P < 0.001), G0-G1 arrest was also noted in both cell types after 72 h in the 20 Gy group (P < 0.001). Although increased apoptosis was observed in both cell lines after irradiation (P < 0.001), a greater percentage of SW480 cells underwent apoptosis in response to irradiation than SW620 cells. Increased Hsp27 and decreased integrin ¦Â₃, Ki67 and VEGFR2 expression was observed over time via immunocytochemistry and Western blot analysis (P < 0.001), however, no significant changes were noted in response to irradiation. Finally, reduced uptake of ¹⁸F-FLT by SW480 or SW620 cells was observed at 24-h post-irradiation, however, reduced ¹⁸F-FDG uptake was only observed after 72 h. Therefore, we conclude that ¹⁸F-FLT is a more suitable positron emission tomography (PET) tracer for monitoring early responses to irradiation in primary and metastatic lymph node CRC cells.