Microsatellite polymorphism in Jatropha curcas L.¡ªA biodiesel plant

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• 作者：Ramanuj Maurya ; Astha Gupta ; Sunil Kumar Singh ; Krishan Mohan Rai ; Ch ; rawati ; Samir V. Sawant ; Hemant Kumar Yadav
• 关键词：Jatropha curcas ; Enriched library ; Genomic SSR ; Biodiesel ; Genetic diversity
• 刊名：Industrial Crops and Products
• 出版年：2013
• 出版时间：August, 2013
• 年：2013
• 卷：49
• 期：Complete
• 页码：136-142
• 全文大小：1224 K

文摘

We developed and characterized 1207 SSRs to enrich the validated markers repertoire of Jatropha curcas. A total of 248 polymorphic SSRs were identified with a panel of 7 accessions of J. curcas including some exotic accessions. Furthermore, 179 and 331 SSRs were found polymorphic among parental lines of NBRI-J05 ¡Á EC643912 and Chhatrapati ¡Á Jatropha integerrima used in developing two mapping population respectively. The number of alleles varied from 2 to 5 with an average of 2.24 ¡À 0.55 and 2.42 ¡À 0.62 alleles/SSR for CA and GA enriched library respectively. Most of the SSRs had lower PIC value (less than 0.30) and the maximum PIC value was observed for JGM_A281, JGM_A326 (0.63) followed by JGM_B300 (0.62), JGM_B361 (0.58), JGM_A244 (0.55), JGM_B595 (0.55) and JGM_B176 (0.55). The genetic similarity coefficient among the accessions of J. curcas and one accession of J. integerrima ranged from 0.11 to 0.92 with an average of 0.57 ¡À 0.31. The phenogram classified all the 7 accessions of J. curcas in one cluster and the J. integerrima remained as an out group. The BLASTX analysis of SSR containing sequences showed maximum similarity of 50 % with Ricinus communis (Euphorbiaceae) followed by Populus trichocarpa (22 % ), Vitis vinifera (16 % ) and Arabidopsis spp. (4.5 % ). This study may enrich the validated repertoire of SSR markers in J. curcas and could be used in various genetic studies including construction of linkage map, diversity analysis, and QTL/association mapping.