Electric field enhanced 3D scalable low-voltage nano-spike electroporation system

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• 作者：Kashif Riaz^a ; ^dAuthor Vitae ; Siu-Fung Leung^bAuthor Vitae ; Zhiyong Fan^bAuthor Vitae ; Yi-Kuen Lee^a ; ^c ; ^{meyklee@ust.hk}Author Vitae
• 关键词：Nano-spike electroporation chips ; Scalable ; Electroporation ; Efficient molecules delivery ; High cell viabilities ; Electric field enhancement ; High-aspect-ratio
• 刊名：Sensors and Actuators A: Physical
• 出版年：2017
• 出版时间：1 March 2017
• 年：2017
• 卷：255
• 期：Complete
• 页码：10-20
• 全文大小：2318 K
• 卷排序：255

文摘

Low-cost 3D scalable efficient nano-spike electroporation system for efficient molecules delivery with high cell viabilities at low voltages. Fabrication of highly ordered 3D nano-spike arrays using simple, low-cost, electrochemical anodization and etching process. Fabrication of 3D NSPs on chips for microsystems as well as on wafer level for large scale high throughput processing. High EP efficiencies and cell viability фcell at much lower voltages (2V) on NSP-EP chips than those of PEP devices. NSP-EP chips minimize undesirable electrochemical reactions and electrolysis that were observed on PEP devices. Low-cost nano-spike electroporation devices enable the integration of mini-electroporator for lab-on-chip systems.