A novel ATP quantification method combining glucose phosphorylation with surface-enhanced Raman scattering

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• 作者：Chun An Shi^a ; ^bAuthor Vitae ; Xin Zhang^a ; ^b ; ^{zhxin@mail.buct.edu.cn}Author Vitae ; Hong Jun Yin^a ; ^bAuthor Vitae ; Hui Fang^a ; ^bAuthor Vitae ; Yong Mei Zhao^cAuthor Vitae ; Luo Liu^aAuthor Vitae ; Zheng Long Wu^dAuthor Vitae ; Hai Jun Xu^a ; ^b ; ^{hjxu@mail.buct.edu.cn}Author Vitae
• 关键词：Adenosine triphosphate (ATP) ; Glucose ; Surface-enhanced Raman scattering (SERS) ; Quantitative determination ; Difference value method
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2017
• 出版时间：31 March 2017
• 年：2017
• 卷：241
• 期：Complete
• 页码：855-859
• 全文大小：800 K
• 卷排序：241

文摘

A novel difference value method combining glucose phosphorylation with surface-enhanced Raman scattering (SERS) for ATP quantification is proposed. The quantitative determination of glucose is achieved based on SERS rather than the traditional spectrophotometry. The proper concentration of glucose for ATP quantification is demonstrated to be 1–1000 times of that of ATP. The accuracy of the difference value method is verified, and the limit of detection of ATP reaches as low as 4.69 × 10−7 M.