A novel electrochemical aptasensor for ultrasensitive detection of fluoroquinolones based on single-stranded DNA-binding protein

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• 作者：Khalil Abnous^a ; ¹Author Vitae ; Noor Mohammad Danesh^b ; ^c ; ¹Author Vitae ; Mona Alibolandi^aAuthor Vitae ; Mohammad Ramezani^bAuthor Vitae ; Seyed Mohammad Taghdisi ; Dr.^d ; ^e ; ^{taghdisihm@mums.ac.ir}Author Vitae ; Ahmad Sarreshtehdar Emrani ; Dr.^f ; ^{ahmad_sarreshtedar@yahoo.cm}Author Vitae
• 关键词：Electrochemical aptasensor ; Fluoroquinolones ; Single-stranded DNA-binding protein ; Gold electrode ; Ciprofloxacin
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：240
• 期：Complete
• 页码：100-106
• 全文大小：1291 K
• 卷排序：240

文摘

Fluoroquinolones (FQs) are vastly applied in human and veterinary medicine to treat a wide scale of infectious diseases. An aptasensor was developed for detection of FQs, based on single-stranded DNA-binding protein (SSB) and gold electrode. The presence of SSB could guarantee limited access of the redox probe to the surface of electrode in the absence of target. The sensor exhibited high specificity toward FQs with a limit of detection (LOD) as low as 263 pM for ciprofloxacin. Moreover, the developed sensing method was successfully applied for determination of ciprofloxacin in serum, milk.