Modeling the effects of mineral nutrition for improving growth and development of micropropagated red raspberries

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• 作者：Sukalya Poothong ; Barbara M. Reed
• 关键词：In vitro culture ; Mineral nutrition ; Response surface methodology ; Rubus ; Red raspberry
• 刊名：Scientia Horticulturae
• 出版年：22 January, 2014
• 年：2014
• 卷：165
• 期：Complete
• 页码：132-141
• 全文大小：3594 K

文摘

In vitro propagation is important for rapid multiplication of a wide range of nursery crops, including red raspberries. The genetic variation of the many red raspberry cultivars makes it difficult to use one growth medium for all. Although some cultivars grow well on medium (MS), others display stunting, hyperhydricity, discoloration, callus, leaf spots, or necrosis. This study used response surface methodology (RSM) to determine the effects of MS mineral salts on red raspberry growth and which of these mineral salts are critical for improving growth. In vitro growth of five red raspberry cultivars was determined by varying five factors that included NH₄NO₃, KNO₃, mesos salts (CaCl₂, KH₂PO₄ and MgSO₄), minor elements (Zn-Mn-Cu-Co-Mo-B-I), and EDTA-chelated iron. The effects of these five factors on plant quality, multiplication, shoot length, leaf size, leaf area, leaf color, callus and leaf spots were determined. The effects varied by cultivar for some characteristics, but all cultivars had improved growth or appearance on some experimental treatments when compared to MS medium. Increased mesos was the most significant factor associated with plant quality, multiplication and shoot length in all cultivars. Increasing iron above MS levels decreased quality in all cultivars except 鈥榃illamette鈥? Decreased KNO₃ with increased mesos and low iron were required to improve shoot multiplication. Increased NH₄NO₃ resulted in greater shoot elongation only in 鈥榃illamette鈥? Determining the driving mineral factors is the first step in improved medium formulations for micropropagated red raspberries.