VEGF₁₆₅ modulates proliferation, adhesion, migration and differentiation of cultured human outer root sheath cells from central hair follicle epithelium through VEGFR-2 activation in vitro

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• 作者：Xian-Jie Wu ; Jian-Wei Zhu ; Jing Jing ; Dan Xue ; Hai Liu ; Min Zheng ; Zhong-Fa Lu
• 关键词：VEGFR ; vascular endothelial growth factor receptor ; NRP ; neuropilin ; ORS ; outer root sheath ; IRS ; inner root sheath ; DP ; hair follicle dermal papilla ; DPC ; hair follicle dermal papilla cell ; DAPI ; 4 ; 6-diamidino-2-phenylindole ; Jun ; jun oncogene ; ERK ; extracellular signal-regulated kinase ; p38 ; p38-mitogen-activated protein kinase ; PLC-c ; phospholipase C-c ; PKC-a ; protein kinase C-a ; RT-PCR ; reverse transcript polymerase chain reaction
• 刊名：Journal of Dermatological Science
• 出版年：February, 2014
• 年：2014
• 卷：73
• 期：2
• 页码：152-160
• 全文大小：2295 K

文摘

Background

The functional state of vasculature is tightly controlled by vascular endothelial growth factor receptor-2 (VEGFR-2). Recent studies revealed that VEGFR-2 is expressed on hair follicle keratinocytes.

Objective

We proposed to investigate its effect on proliferation, adhesion and migration of cultured human outer root sheath cells from central hair follicle epithelium.

Methods

These studies were undertaken in vitro using human outer root sheath cells from central hair follicle epithelium, immunohistochemistry analysis, immunofluorescence microscopy, western blot analysis, MTT, trans well analysis, and RT-PCR.

Results

Our results show that VEGFR-2 is expressed in these cells in vivo and in vitro. Furthermore, proliferation and migration of cultured human outer root sheath cells from central hair follicle epithelium is increased by VEGF₁₆₅, while homotypic adhesion is decreased but heterotypic adhesion is increased. VEGF₁₆₅ upregulates integrin 尾1 but dowregulates lgr6 expression. In addition, phosphorylation of VEGFR-2, Erk1/2, c-Jun and p38, are increased following VEGF₁₆₅ treatment and these effects are reversed by a VEGFR-2 neutralizing antibody.

Conclusion

Our results suggest a role of VEGF/VEGFR-2 beyond angiogenesis in hair follicle regulation.