Hydrogen sulfide promotes proliferation and neuronal differentiation of neural stem cells and protects hypoxia-induced decrease in hippocampal neurogenesis

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• 作者：Dexiang Liu ; Zhen Wang ; Jingmin Zhan ; Qun Zhang ; Jianmei Wang ; Qingrui Zhang ; Xiuying Xian ; Qinsong Luan ; Aijun Hao
• 关键词：Hydrogen sulfide ; Neural stem cell ; Neurogenesis ; Hypoxia
• 刊名：Pharmacology Biochemistry and Behavior
• 出版年：January, 2014
• 年：2014
• 卷：116
• 期：Complete
• 页码：55-63
• 全文大小：951 K

文摘

Accumulating evidence has suggested that hydrogen sulfide (H₂S) acts as a novel neuro-modulator and neuroprotective agent; however, it remains to be investigated whether H₂S has a direct effect on neural stem cells (NSCs). In the present study, we examined the effects of H₂S donor, sodium hydrosulfide (NaHS) on mouse NSCs and hippocampal neurogenesis. We report here that NaHS promoted proliferation and neuronal differentiation of NSCs. Further analysis revealed that NaHS-induced proliferation was associated with phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 and neuronal differentiation was linked to altered expression of differentiation-related genes. In addition, C57BL/6 mice (1 day old) subjected to hypoxia were treated with NaHS to explore whether H₂S would influence the neurogenesis of hippocampus. BrdU incorporation assay results showed that administration of NaHS could increase the number of proliferating cells in the dentate gyrus of hippocampus in the mice after hypoxia. Moreover, Morris water maze test showed that treatment with NaHS improved cognitive impairment after hypoxia in mice. These findings suggest that H₂S may afford a novel therapeutic strategy to intervene in the progression of brain diseases.