2,3,5,4鈥?tetrahydroxystilbene-2-O-尾-D-glucoside (THSG) attenuates human platelet aggregation, secretion and spreading in vitro

详细信息    查看全文

• 作者：Ke Xiang ; Gang Liu ; Ya-Jun Zhou ; Hong-Zhen Hao ; Zhao Yin ; Ao-Di He ; Xing-Wen Da ; Ji-Zhou Xiang ; Jia-Ling Wang ; Zhang-Yin Ming
• 关键词：THSG ; 2 ; 3 ; 5 ; 4&rsquo ; -tetrahydroxystilbene-2-O-尾-D glucoside ; PRP ; platelet rich plasma ; PPP ; platelet poor plasma ; U46619 ; 9 ; 11-Dideoxy-9a ; 11a-methanoepoxy prostaglandin F2a ; TxA2 ; thromboxaneA2 ; ADP ; adenosine diphosphate ; ATP ; adenosine triphosphate ; PGE1 ; Prostaglandin E1 ; EDTA ; ethyle nediaminetetraacetic acid ; MAPK ; mitogen ; activated protein kinase
• 刊名：Thrombosis Research
• 出版年：February, 2014
• 年：2014
• 卷：133
• 期：2
• 页码：211-217
• 全文大小：1147 K

文摘

Introduction

2,3,5,4鈥?tetrahydroxystilbene-2-O-尾-D-glucoside(THSG) is a water-soluble component of the rhizome extract from the traditional Chinese herb Polygonum multiflorum. Recent studies have demonstrated that THSG has potent anti-oxidative and anti-inflammatory effects. In this study, we investigated the anti-platelet aggregation, secretion and spreading of THSG with different methods. The purpose was to explore the anti-platelet effect of THSG and the underlying mechanism.

Materials and Methods

We investigated the anti-platelet activity of THSG on platelet aggregation induced by collagen (2 渭g/mL), thrombin(0.04U/mL), U46619 (3 渭M) and ADP (2 渭M). ATP secretion induced by collagen (2 渭g/mL) was also investigated. P-selectin expression and PAC-1 binding were measured by flow cytometry. In addition, human platelet spreading on immobilized fibrinogen and immunoblotting were also tested.

Results

THSG dose-dependently inhibited platelet aggregation and ATP secretion induced by collagen. It inhibited platelet P-selectin expression and PAC-1 binding induced by thrombin(0.1U/mL). THSG also inhibited human platelet spreading on immobilized fibrinogen, a process mediated by platelet outside-in signaling. Western blot analysis showed that THSG could inhibit platelet Fc 纬 RIIa, Akt(Ser473)and GSK3尾(Ser9) phosphorylation.

Conclusions

Our study indicates that THSG has potent anti-platelet activity to collagen induced aggregation. THSG is likely to exert protective effects in platelet-associated thromboembolic disorders by modulating human platelet.