Highly sensitive detection of mercury ion based on T-rich DNA machine using portable glucose meter

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• 作者：Shan Huang^a ; ^bAuthor Vitae ; Wenjing Wang^bAuthor Vitae ; Fangfang Cheng^cAuthor Vitae ; Huiqin Yao^a ; ^{huiqin_yao@163.com}Author Vitae ; Jun-Jie Zhu^b ; ^{jjzhu@nju.edu.cn}Author Vitae
• 关键词：Mercury ion ; T-rich DNA machine ; Nicking enzyme-assisted signal amplification ; Portable glucose meter
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2017
• 出版时间：April 2017
• 年：2017
• 卷：242
• 期：Complete
• 页码：347-354
• 全文大小：1906 K
• 卷排序：242

文摘

The proposed method could realize detection of Hg2+ at low femtomolar concentration. In this method, mercury could triggering the T-rich DNA machine to produce a large amount of product DNA for signal amplification. The output PGM signals which came from glucose is related with the amount of product DNA, indirectly quantifying the concentration of Hg2+. The PGM is expected to have a wide application in routine detection the concentration of Hg2+ in environmental water and biologic samples in everyone’s home.