Formation of long-lived reactive species of blood serum proteins by the action of heat

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• 作者：Vadim I. Bruskov ; Nelly R. Popova ; Vladimir E. Ivanov ; Olga E. Karp ; Anatoly V. Chernikov ; Sergey V. Gudkov
• 关键词：BGG ; bovine gamma-globulin ; BSA ; bovine serum albumin ; CCA ; coumarin-3-carboxylic acid ; LRPS ; long-lived reactive protein species ; PBS ; phosphate-buffered saline ; ROS ; reactive oxygen species ; SOD ; superoxide dismutase
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：17 January, 2014
• 年：2014
• 卷：443
• 期：3
• 页码：957-961
• 全文大小：359 K

文摘

It has been previously established that heat induces the formation of reactive oxygen species (ROS) in aqueous solutions. In biological systems, ROS cause oxidative damage predominantly to proteins due to their abundance and sensitivity to oxidation. Proteins oxidized by the action of X-rays represent long-lived reactive species, which trigger the secondary generation of ROS (Bruskov et al. (2012) ). Here we studied the possibility of formation of long-lived species of the blood serum proteins bovine serum albumin and bovine gamma-globulin in air-saturated solutions under the action of heat. It is shown that heat induces the generation of long-lived protein species, which in turn generate ROS (¹袨₂, , O袧, and H₂O₂). The formation of the long-lived reactive species of BSA and BGG with a half-life of about 4 h induced by moderate hyperthermia was revealed using the chemiluminescence of protein solutions. It was found that long-lived reactive species of BSA and BGG cause prolonged generation of H₂O₂. The results obtained suggest that H₂O₂ produced by proteins after heating represents a messenger in signaling pathways and produces therapeutic effects in living organisms.