Two novel analytical methods based on polyclonal and monoclonal antibodies for the rapid detection of Cronobacter spp.: Development and application in powdered infant formula

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• 作者：Xin Xu ; Yan Zhang ; Man Shi ; Wei Sheng ; Xinjun Du ; Meng Yuan ; Shuo Wang
• 关键词：Cronobacter spp. ; Monoclonal antibody ; ELISA ; Infant formula
• 刊名：Lebensmittel-Wissenschaft und -Technologie
• 出版年：May, 2014
• 年：2014
• 卷：56
• 期：2
• 页码：335-340
• 全文大小：471 K

文摘

Cronobacter spp. are opportunistic pathogens, and infections are associated with a high mortality rate. In the current study, monoclonal antibodies (mAbs) and polyclonal antibodies (pAbs) were generated using heat-inactivated C.聽sakazakii strain ATCC29544 as the immunogen. Following assay optimization, an indirect enzyme-linked immunosorbent assay (ELISA) based on pAbs and a sandwich ELISA based on mAbs and pAbs were established for the detection of Cronobacter spp. The indirect ELISA detected all species of Cronobacter assayed, and the limit of detection (LOD) was established as 10⁵聽cfu/mL. In contrast, the sandwich ELISA was specific for C.聽sakazakii and had greater sensitivity than the indirect ELISA (LOD of 2聽脳聽10⁴聽cfu/mL). Following 10聽h of enrichment, Cronobacter spp. were detected using either of the two analytical methods in samples inoculated with 1聽cfu/100聽g powdered infant formula (PIF). The results from this study demonstrated that both of these novel ELISAs were specific, sensitive, and rapid assays for the screening of pathogenic Cronobacter spp. in PIF.