Mitochondria-targeted ratiometric fluorescent probe based on FRET for bisulfite

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• 作者：Wen-Li Wu^a ; ¹Author Vitae ; Han-Lin Ma^b ; ¹Author Vitae ; Miao-Fei Huang^aAuthor Vitae ; Jun-Ying Miao^b ; ^{miaojy@sdu.edu.cn}Author Vitae ; Bao-Xiang Zhao^a ; ^{bxzhao@sdu.edu.cn} ; ^{sduzhao@hotmail.com}Author Vitae
• 关键词：Fluorescence ; Ratiometric ; Mitochondria-targeted ; Bisulfite ; Cell imaging
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2017
• 出版时间：31 March 2017
• 年：2017
• 卷：241
• 期：Complete
• 页码：239-244
• 全文大小：1687 K
• 卷排序：241

文摘

A ratiometric mitochondria-targeted fluorescent probe DSPT for bisulfate was developed based on FRET mechanism. DSPT has competitive limit of detection (69 nM). DSPT was applied to detect exogenous and endogenous bisulfite in living Hela and HepG2 cells. DSPT was also successful applied to discriminate liver cancer cells from normal liver cells.