A conserved region in the Closterovirus 1a polyprotein drives extensive remodeling of endoplasmic reticulum membranes and induces motile globules in Nicotiana benthamiana cells

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• 作者：V.A. Gushchin^a ; ^b ; D.G. Karlin^c ; A.V. Makhotenko^a ; A.V. Khromov^a ; T.N. Erokhina^d ; A.G. Solovyev^e ; ^f ; S.Yu. Morozov^e ; A.A. Agranovsky^a ; ^g ; ^{aaa@genebee.msu.su}
• 关键词：RNA virus replication ; Membrane vesicles ; Virus replication factory ; Endoplasmic reticulum modification
• 刊名：Virology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：502
• 期：Complete
• 页码：106-113
• 全文大小：1251 K
• 卷排序：502

文摘

In infected plant cells, closterovirus replicative polyproteins 1a and 1ab drive membrane remodeling and formation of multivesicular replication platforms. Polyprotein 1a contains a variable Central Region (CR) between the methyltransferase and helicase domains. In a previous study, we have found that transient expression of the Beet yellows virus CR-2 segment (aa 1305-1494) in Nicotiana benthamiana induces the formation of ~1 µm mobile globules originating from the ER membranes. In the present study, sequence analysis has shown that a part of the CR named the “Zemlya region” (overlapping the CR-2), is conserved in all members of the Closterovirus genus and contains a predicted amphipathic helix (aa 1368-1385). By deletion analysis, the CR-2 region responsible for the induction of 1-μm globules has been mapped to aa 1368-1432. We suggest that the conserved membrane-modifying region of the BYV 1a may be involved in the biogenesis of closterovirus replication platforms.