An array fluorescent biosensor based on planar waveguide for multi-analyte determination in water samples

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• 作者：Lanhua LiuAuthor Vitae ; Xiaohong Zhou ; ^{xhzhou@mail.tsinghua.edu.cn}Author Vitae ; Mifang LuAuthor Vitae ; Mingkai ZhangAuthor Vitae ; Chao YangAuthor Vitae ; Ruiman MaAuthor Vitae ; Abdul Ghaffar MemonAuthor Vitae ; Hanchang ShiAuthor Vitae ; Yi QianAuthor Vitae
• 关键词：Array immunosensor ; Planar waveguide ; Evanescent wave ; Fluorescence ; Binding kinetics
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：240
• 期：Complete
• 页码：107-113
• 全文大小：869 K
• 卷排序：240

文摘

A planar waveguide-based array immunosensor (PWAI) was described, allowing measurements of up to twenty-four analytes in eight separate channels rapidly, sensitively and simultaneously. In this system, a linear laser light created by a line generator was coupled into a planar optical waveguide via a beveled angle, forming eight individual total internal reflection (TIR) lines. A multi-channel microfluidics cell was employed to isolate the parallel TIR lines physically so as to form eight independent flow channels on the same chip, avoiding the cross-reactivity of antibodies and supporting various bioassay conditions. By employing fluorescent detection with fluorophore-labeled antibodies binding to the surface of the waveguide with the analyte derivative covalently attached, the array immunoassays can realize the multi-analyte biosensing. A model was proposed to guide the design of such a planar waveguide-based evanescent wave biosensor. The proposed system was confirmed with the comparable sensitivity with previously reported waveguide biosensor. In addition to being a multi-channel analytical device for the highly sensitive detection of contaminants, the proposed evanescent wave PWAI can provide the dynamic surface-based biomolecular interaction information regarding the affinity and kinetics with high sensitivity.