Polymerase chain reaction with nearby primers

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• 作者：Ravil R. Garafutdinov ; ^{garafutdinovr@gmail.com} ; Aizilya A. Galimova ^{aiz.galimova@yandex.ru} ; Assol R. Sakhabutdinova ^{sakhabutdinova@rambler.ru}
• 关键词：Polymerase chain reaction ; Amplification ; Nearby primers ; Abutting primers ; PCR specificity ; PCR sensitivity ; Degraded DNA
• 刊名：Analytical Biochemistry
• 出版年：2017
• 出版时间：1 February 2017
• 年：2017
• 卷：518
• 期：Complete
• 页码：126-133
• 全文大小：1583 K
• 卷排序：518

文摘

DNA analysis of biological specimens containing degraded nucleic acids such as mortal remains, archaeological artefacts, forensic samples etc. has gained more attention in recent years. DNA extracted from these samples is often inapplicable for conventional polymerase chain reaction (PCR), so for its amplification the nearby primers are commonly used. Here we report the data that clarify the features of PCR with nearby and abutting primers. We have shown that the proximity of primers leads to significant reduction of the reaction time and ensures the successful performance of DNA amplification even in the presence of PCR inhibitors. The PCR with abutting primers is usually characterized by the absence of nonspecific amplification products that causes extreme sensitivity with limit of detection on single copy level. The feasibility of PCR with abutting primers was demonstrated on species identification of 100 years old rotten wood.