Sequential immunization with consensus influenza hemagglutinins raises cross-reactive neutralizing antibodies against various heterologous HA strains

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• 作者：Hui Zhou^a ; ^b ; ¹ ; Yang Huang^c ; ¹ ; Songhua Yuan^d ; Yuanyuan Li^a ; Shuyan Wu^a ; Jianqing Xu^d ; ^{xujianqing@shphc.org.cn} ; Rui Huang^a ; ^{hruisdm@163.com} ; ^{huangruisuda@aliyun.com}
• 关键词：nAb ; neutralizing antibodies ; MDCK ; Madin-Darby canine kidney ; TCID50 ; tissue culture infectious dose ; SPF ; specific pathogen free ; i.m. ; intramuscularly ; ELISA ; enzyme-linked immunosorbent assay ; ELISPOT ; enzyme-linked immunosorbent spot ; RBC ; red blood cells ; RLU ; relative luminescence units ; HAI ; hemagglutination inhibition ; RDE ; receptor-destroying enzyme ; IgG ; immunoglobulin G ; OD ; optical density
• 刊名：Vaccine
• 出版年：2017
• 出版时间：5 January 2017
• 年：2017
• 卷：35
• 期：2
• 页码：305-312
• 全文大小：1569 K
• 卷排序：35

文摘

Sequential vaccination with heterologous HA elicit more uniformed T-cell responses. Sequential vaccination with heterologous HA elicit more broader cross-reactive nAb. Broad monoclonal nAb could be isolated form sequential vaccinated mice. An universal vaccine is possible by employ the sequential vaccination strategy.