Cloning, characterization, hypoxia and heat shock response of hypoxia inducible factor-1 (HIF-1) from the small abalone Haliotis diversicolor

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• 作者：Xiuhong Cai ; Yitao Huang ; Xin Zhang ; Shuhong Wang ; Zhihua Zou ; Guodong Wang ; Yilei Wang ; Ziping Zhang
• 关键词：HIF-1伪 ; hypoxia inducible factor-1伪 ; HIF-1尾 ; hypoxia inducible factor-1尾 ; ARNT ; aryl hydrocarbon receptor nuclear translocator ; bHLH/PAS ; basic helix&ndash ; loop&ndash ; helix/Per-Arnt-Sim ; CBP ; CREB1-binding protein ; C-TAD ; C-terminal transactivation domain ; DO ; dissolved oxygen ; HRE ; hypoxia response element ; NRR ; neutral red retention ; NRU ; neutral red uptake ; N-TAD ; N-terminal transactivation domain ; ODDD ; oxygen dependent degradation domain ; ORF ; open reading frame ; PHDs ; proline hydroxyla
• 刊名：Gene
• 出版年：25 January, 2014
• 年：2014
• 卷：534
• 期：2
• 页码：256-264
• 全文大小：3316 K

文摘

In this study, hypoxia inducible factor-1伪 (HIF-1伪) and hypoxia inducible factor-1尾 (HIF-1尾) from small abalone Haliotis diversicolor were cloned. The cDNA of H. diversicolor HIF-1伪 (HdHIF-1伪) is 2833 bp encoding a protein of 711aa and H. diversicolor HIF-1尾 (HdHIF-1尾) is 1919 bp encoding a protein of 590aa. Similar to other species' HIF-1, HdHIF-1 has one basic helix-loop-helix (bHLH) domain and two Per-Arnt-Sim (PAS) domains, and HdHIF-1伪 has a oxygen-dependent degradation domain (ODDD) with two proline hydroxylation motifs and a C-terminal transactivation domain (C-TAD) with an asparagine hydroxylation motif. Under normoxic conditions, HdHIF-1伪 and HdHIF-1尾 mRNAs were constitutively present in all examined tissues. Under hypoxia (2.0 mg/L DO at 25 掳C) stress, HdHIF-1伪 expression was up-regulated in gills at 4 h, 24 h and 96 h, and in hemocytes at 24 h and 96 h, while HdHIF-1尾 remained relatively constant. Under thermal stress (31 掳C), HdHIF-1伪 expression was significantly increased in gills at 4 h, and hemocytes at 0 h and 4 h, while HdHIF-1尾 expression still remained relatively constant. These results suggested that HIF-1伪 may play an important role in adaption to poor environment in H. diversicolor.