Shaken or stirred?: Comparison of methods for dispersion of Mycoplasma pneumoniae aggregates for persistence in vivo

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• 作者：Arthur H. Totten^a ; ^{ahtotten@uab.edu} ; Li Xiao^b ; ^{lixiao@uabmc.edu} ; Donna M. Crabb^c ; ^{dcrabb@uabmc.edu} ; Amy E. Ratliff^c ; ^{amyratliff@uabmc.edu} ; Kevin Dybvig^d ; ^{dybvig@uab.edu} ; Ken B. Waites^c ; ^{kwaites@uabmc.edu} ; T. Prescott Atkinson^a ; ^{PAtkinson@peds.uab.edu}
• 关键词：Mycoplasma pneumoniae ; Mollicutes ; Sonication ; Dispersion ; Bacterial persistence
• 刊名：Journal of Microbiological Methods
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：132
• 期：Complete
• 页码：56-62
• 全文大小：944 K
• 卷排序：132

文摘

Sonication is superior to alternative forms of disruption for M. pneumoniae. Adherent mycoplasmas may benefit the most from sonication. Sonicated preparations exhibited increased persistence in vivo.