DNA methyltransferase activity detection based on graphene quantum dots using fluorescence and fluorescence anisotropy

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• 作者：Hanie Ahmadzade Kermani^aAuthor Vitae ; Morteza Hosseini^a ; ^{smhosseini@khayam.ut.ac.ir}Author Vitae ; Mehdi Dadmehr^bAuthor Vitae ; Saman Hosseinkhani^cAuthor Vitae ; Mohammad Reza Ganjali^d ; ^eAuthor Vitae
• 关键词：DNA methyltransferase activity ; Graphene quantum dots ; Fluorescence ; Anisotropy
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2017
• 出版时间：31 March 2017
• 年：2017
• 卷：241
• 期：Complete
• 页码：217-223
• 全文大小：1565 K
• 卷排序：241

文摘

GQDs has been developed as a fluorescence probe for M.SssI MTase activity assay. Detection of MTase activity is also investigated by fluorescence anisotropy. The method shows acceptable selectivity and could detect target in real biological matrix. The detection limit of this assay is 0.7 U/ml. The method is low cost and easy prepare and can be used to detect other MTase activity by changing DNA sequences.