A wireless and sensitive detection of octachlorostyrene using modified AuNPs as signal-amplifying tags

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• 作者：Lan Chen ; Jiezhen Li ; T.Tran ThanhThuy ; Liping Zhou ; Chen'an Huang ; Lijuan Yuan ; Qingyun Cai
• 关键词：Octachlorostyrene ; Wireless ; Immunosensor ; AuNPs ; Amplify
• 刊名：Biosensors and Bioelectronics
• 出版年：15 February, 2014
• 年：2014
• 卷：52
• 期：Complete
• 页码：427-432
• 全文大小：974 K

文摘

A wireless, remote query octachlorostyrene (OCS) biosensor was fabricated by coating a mass-sensitive magnetoelastic ribbon with anti-OCS antibody. In response to a time-varying magnetic field, the magnetoelastic sensor mechanically vibrates at a characteristic resonance frequency which inversely depends on the sensor mass loading. As the magnetoelastic film is magnetostrictive itself, the vibrations launch magnetic flux that can be remotely detected using a pickup coil. Au nanoparticles (NPs) were used to amplify the mass loading. In a sample solution containing OCS target and OCS-modified AuNPs (OCS-AuNPs), both OCS and OCS-AuNPs react with the anti-OCS antibody immobilized on the sensor surface in a competition mode. The bound OCS-AuNPs amount is inversely proportional to the OCS target concentration. The reduction of bound OCS-AuNPs induced by free OCS results in significant change in mass loading, which amplifies the responses. The biosensor demonstrates a linear shift in resonance frequency with OCS concentration between 7.4 渭M and 9 nM, with a detection limit of 2.8 nM.