Leucine-rich repeat-containing G-protein-coupled receptor 5-positive cells in the endometrial stem cell niche

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• 作者：Irene Cervelló ; Ph.D.^a ; ^{irene.cervello@ivi.es} ; Claudia Gil-Sanchis ; Ph.D.^a ; Xavier Santamarí ; a ; Ph.D. ; M.D.^a ; ^b ; ^c ; Amparo Faus ; B.Sc.^a ; Julia Vallvé ; -Juanico ; Ph.D.^b ; ^c ; Patricia Dí ; az-Gimeno ; Ph.D.^a ; Oriana Genolet ; Ph.D.^a ; Antonio Pellicer ; Ph.D. ; M.D.^a ; Carlos Simó ; n ; Ph.D. ; M.D.^a ; ^d
• 关键词：Somatic stem cells ; human endometrium ; stem cell marker ; LGR5 ; hematopoietic lineage
• 刊名：Fertility and Sterility
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：107
• 期：2
• 页码：510-519.e3
• 全文大小：3580 K
• 卷排序：107

文摘

To study, isolate and characterize leucine-rich repeat–containing heterotrimeric guanine nucleotide–binding protein–coupled receptor 5 (LGR5)–positive cells from human endometrium to determine their functional relevance.DesignProspective experimental animal study.SettingUniversity research laboratories.Animal(s)Nonobese diabetic mice (NOD-SCID) (strain code 394; NOD.CB17—Prkdcscid/NcrCrl).Intervention(s)Human LGR5+ cells were labeled with superparamagnetic iron oxide nanoparticles (SPIOs) and injected under the kidney capsule in immunocompromised mice.Main Outcome Measure(s)Epithelial and stromal LGR5+ cells were isolated from human endometrium by means of fluorescence-activated cell sorting, and phenotypic characterization was performed by means of flow cytometry with the use of hematopoietic and mesenchymal markers. Engrafted SPIO-labeled LGR5+ cells were localized with the use of Prussian blue staining and immunohistochemistry against CD9 and Vimentin. Deep transcriptomic profiling of LGR5+ cells was performed with the use of microarrays and RNA sequencing.Result(s)The percentage of LGR5+ cells in human endometrium represented 1.08 ± 0.73% and 0.82 ± 0.76% of total cells in the epithelial and stromal compartments, respectively. LGR5+ cells were phenotypically characterized by abundant expression of CD45 hematopoietic marker and no expression of surface markers CD31, CD34, CD133, CD73, and CD90. Coexpression with the macrophage marker CD163 was detected. Xenotransplantation of labeled LGR5+ cells into the kidney capsules of immunocompromised mice resulted in a weak endometrial reconstitution from this cell of origin. Transcriptomic profiling revealed new attributes for LGR5+ cells related to their putative hematopoietic origin.Conclusion(s)These data suggest that endometrial LGR5 is not an endogenous stem cell marker. Instead, LGR5+ cells appear to be recruited from blood to be part of the stem cell niche at the perivascular microenvironment to activate the endogenous niche.