Applications of genome editing by programmable nucleases to the metabolic engineering of secondary metabolites

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• 作者：Ana Lú ; cia Leitã ; o^a ; ^b ; ^{aldl@fct.unl.pt} ; Marina C. Costa^c ; Francisco J. Enguita^c ; ^{fenguita@fm.ul.pt}
• 关键词：Secondary metabolism ; Genome editing ; TALEN ; CRISPR-Cas9 ; Hybrid metabolites ; Epigenome editing
• 刊名：Journal of Biotechnology
• 出版年：2017
• 出版时间：10 January 2017
• 年：2017
• 卷：241
• 期：Complete
• 页码：50-60
• 全文大小：2273 K
• 卷排序：241

文摘

Programmable nucleases are efficient tools for genome engineering and synthetic biology applications. Genome editing can be applied to secondary metabolism engineering using forward and reverse genetics protocols. Epigenome editing is a promising technology for discovery and production improvement of secondary metabolites.