Tear Fluid Protein Changes in Dry Eye Syndrome Associated with Rheumatoid Arthritis: A Proteomic Approach

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• 作者：Saijyothi Venkata Aluru ; PhD¹ ; Agarwal Shweta ; MBBS DO² ; Srinivasan Bhaskar ; MS² ; Krishnan Geetha ; MS² ; Rajappa M. Sivakumar ; MD³ ; Tatu Utpal ; PhD⁴ ; Prema Padmanabhan ; MS² ; Narayanasamy Angayarkanni ; PhD¹ ; ^{drak@snmail.org} ; ^{angayar07@gmail.com}
• 关键词：2D-DIGE ; clinical proteomics ; dry eye syndrome ; mass spectrometry ; rheumatoid arthritis ; Sjö ; gren syndrome ; tear proteomics
• 刊名：The Ocular Surface
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：15
• 期：1
• 页码：112-129
• 全文大小：3282 K
• 卷排序：15

文摘

Sjögren syndrome (SS) secondary to rheumatoid arthritis (RA) affects lacrimal and salivary glands, and therefore dry eye syndrome (DES) is more prevalent in patients with RA. This study used a proteomic approach to identify potential biomarkers in tear of DES secondary to RA (DES-RA).MethodsTear specimens were collected with Schirmer strips from patients with DES with RA, patients with other types of dry eye (namely, primary Sjögrens and non-Sjögrens [NSS]), and age-matched controls. Tear proteins were subjected to 2D-differential gel electrophoresis (2D-DIGE), and the differentially expressed proteins were identified using nano ESI-LC-MS/MS analysis.ResultsAmong the differentially regulated proteins of DES-RA that were identified, lactotransferrin isoform 1 precursor was found to be d own-regulated in 100% cases and SHC transforming 1 isoform in 63% of the cases, while proteins such as ribonuclease p protein subunit 20, protocadherin, and heterogeneous nuclear ribonucleoprotein Q isoform 6 were down-regulated in over 80% of the cases. Proteins such as Ecto-ADP ribosyltransferase 5 precursor, Rho-related GTP-binding protein, and RhoJ precursor were up-regulated in 80% of the cases.ConclusionFunctional annotation revealed that these proteins have roles in regulation, antimicrobial activity, immune, metabolic, and cellular processes. The study observed characteristic marker proteins differentially expressed in DES-RA that are previously unreported. Further validation is needed.