Dengue-4 false negative results by Panbio^庐 Dengue Early ELISA assay in Brazil

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• 作者：Tatiana Elias Colombo ; Danila Vedovello ; Carlos Shigueru Araki ; Hugo Cogo-Moreira ; Izalco Nuremberg Penha dos Santos ; Andr茅ia Francesli Negri Reis ; Fabiana Rodrigues Costa ; Lilian Elisa Ar茫o Ant么nio Cruz ; Liane Casagr ; e ; Livia Jos茅 Regatieri ; Jur ; ir Ferreira Junior ; Roberta Vieira de Morais Bronzoni ; Diane J. Schmidt ; Maur铆cio Lacerda Nogueira
• 关键词：Dengue ; NS1 Ag rapid assay ; False negative results
• 刊名：Journal of Clinical Virology
• 出版年：December, 2013
• 年：2013
• 卷：58
• 期：4
• 页码：710-712
• 全文大小：424 K

文摘

Background

Dengue is a serious public health problem in numerous countries. The ability to rapidly diagnosis dengue is important for patient triage and management. Detection of dengue viral protein, NS1, represents a new approach to dengue diagnosis.

Objective

The present study aims to evaluate if there are false negative results using the NS1 Ag rapid assay (Panbio^庐 Dengue Early ELISA) in two different epidemiological situations (epidemic and non-epidemic).

Study design

220 serum samples from patients with clinical symptoms of classical dengue fever were tested by NS1 antigen capture ELISA and Multiplex-Nested-PCR.

Results

In samples collected in a non-epidemic period we found a 100% agreement of ELISA and RT-PCR in dengue negative samples and 85% agreement of ELISA and RT-PCR in dengue positive samples. But when we tested samples during an epidemic period (large DENV-4 outbreak) we found 15% false negative results (p < 0.05) in dengue negative samples.

Conclusions

Due to false negative results for DENV-4, the sole use of the Panbio^庐 Dengue Early ELISA assay as a screening method for monitoring circulating dengue serotypes must be reevaluated.