Detection of Anaplasma platys in dogs using real-time loop-mediated isothermal amplification

详细信息    查看全文

• 作者：Hua-tao Li¹ ; Ling-suang Sun¹ ; Zhong-ming Chen ; Jing-si Hu ; Cun-dong Ye ; Kun Jia ; Heng Wang ; Li-guo Yuan ; Gui-hong Zhang ; Shoujun Li ; ^{shoujunli@scau.edu.cn" class="auth_mail}
• 关键词：Canine ; Anaplasma platys ; gltA gene ; Real-time loop-mediated isothermal amplification
• 刊名：The Veterinary Journal
• 出版年：March, 2014
• 年：2014
• 卷：199
• 期：3
• 页码：468-470
• 全文大小：962 K

文摘

Anaplasma platys is a parasite of canine platelets that causes infectious cyclic thrombocytopenia. In this study, a novel real-time loop-mediated isothermal amplification (RT-LAMP) method was developed to detect A. platys. RT-LAMP primer sets were designed using a citrate synthase gene sequence and the assay was performed at 63 掳C for 30 min. No cross-reactivity was observed with other Anaplasma or Ehrlichia spp. and the method exhibited a similar level of sensitivity in detecting the organism in 58 canine blood samples to that of a nested PCR. This RT-LAMP is a rapid and potentially cost-effective method of diagnosing A. platys infection in dogs.