The leaf extract of Mallotus japonicus and its major active constituent, rutin, suppressed on melanin production in murine B16F1 melanoma

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• 作者：Junsei Taira¹ ; ^{taira@okinawa-ct.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Eito Tsuchida¹ ; Masatsugu Uehara¹ ; Natsuko Ohhama¹ ; Wakana Ohmine¹ ; Takayuki Ogi²
• 关键词：Mallotus japonicus ; Melanogenesis ; Tyrosinase ; Melanin ; Polyphenol ; Rutin
• 刊名：Asian Pacific Journal of Tropical Biomedicine
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：5
• 期：10
• 页码：819-823
• 全文大小：546 K

文摘

To find anti-melanogenesis materials used in whitening cosmetics.

Methods

The ethanolic leaf extract of Mallotus japonicus (M. japonicus) having an anti-melanogenesis activity was separated by a sephadex LH-20 chromatography. Each fraction was measured for its tyrosinase inhibitory activity together with its polyphenol content using the Folin–Ciocalteu method. The anti-melanogenesis activity of the active fractions was determined by the melanin content in the murine B16F1 melanoma. The active fractions were put together due to similar constituents, and then separated by high performance liquid chromatography using a C-18 ODS column. The major anti-melanogenesis compound was identified using ¹H and ¹³C-NMR and liquid chromatography-mass spectrometry.

Results

The ethanolic leaf extract of M. japonicus showed an anti-tyrosinase activity with a high polyphenol content, resulting in suppression of melanin production in the B16F1 melanoma. The extract was separated and the active compound was identical as rutin based on the ¹H, ¹³C-NMR and liquid chromatography-mass spectrometry analysis data. In addition, the rutin treatment with cells reduced the melanin content in a concentration dependent manner without any cell toxicity. The leaf extract of M. japonicus containing rutin would be useful in whitening cosmetics for protection from UV-light exposure to be limiting the accumulation of melanin in skin.

Conclusions

The leaf extract of M. japonicus and/or rutin isolated from the extract as a key whitening agent would be useful as a whitening cosmetic material for protecting against disorder skin due to melanin accumulation.