Induced over-expression of AtDREB2A CA improves drought tolerance in sugarcane

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• 作者：Rafaela Ribeiro Reis^a ; ^b ; ¹ ; Bá ; rbara Andrade Dias Brito da Cunha^a ; ¹ ; Polyana Kelly Martins^a ; Maria Thereza Bazzo Martins^a ; Jean Carlos Alekcevetch^b ; Antô ; nio Chalfun-Jú ; nior^b ; Alan Carvalho Andrade^c ; Ana Paula Ribeiro^a ; ^b ; Feng Qin^d ; ² ; Junya Mizoi^e ; Kazuko Yamaguchi-Shinozaki^e ; Kazuo Nakashima^d ; Josirley de Fá ; tima Corrê ; a Carvalho^f ; Carlos Antô ; nio Ferreira de Sousa^a ; Alexandre Lima Nepomuceno^f ; Adilson Kenji Kobayashi^a ; Hugo Bruno Correa Molinari^a ; ^{hugo.molinari@embrapa.br" class="auth_mail} ; ^{molinari.hugo@gmail.com" class="auth_mail}
• 关键词：ABA ; abscisic acid ; DREB ; DRE-binding protein ; DREB2A ; DRE-binding protein 2A ; DRE ; dehydration-responsive element ; AP2/ERF ; APETALA2/ethylene responsive-element binding factor
• 刊名：Plant Science
• 出版年：May, 2014
• 年：2014
• 卷：221-222
• 期：Complete
• 页码：59-68
• 全文大小：2236 K

文摘

Drought is one of the most challenging agricultural issues limiting sustainable sugarcane production and, in some cases, yield losses caused by drought are nearly 50%. DREB proteins play vital regulatory roles in abiotic stress responses in plants. The transcription factor DREB2A interacts with a cis-acting DRE sequence to activate the expression of downstream genes that are involved in drought-, salt- and heat-stress response in Arabidopsis thaliana. In the present study, we evaluated the effects of stress-inducible over-expression of AtDREB2A CA on gene expression, leaf water potential (唯_L), relative water content (RWC), sucrose content and gas exchanges of sugarcane plants submitted to a four-days water deficit treatment in a rhizotron-grown root system. The plants were also phenotyped by scanning the roots and measuring morphological parameters of the shoot. The stress-inducible expression of AtDREB2A CA in transgenic sugarcane led to the up-regulation of genes involved in plant response to drought stress. The transgenic plants maintained higher RWC and 唯_L over 4 days after withholding water and had higher photosynthetic rates until the 3rd day of water-deficit. Induced expression of AtDREB2A CA in sugarcane increased sucrose levels and improved bud sprouting of the transgenic plants. Our results indicate that induced expression of AtDREB2A CA in sugarcane enhanced its drought tolerance without biomass penalty.