High expression of heat shock protein 90 alpha and its significance in human acute leukemia cells

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• 作者：Wen-Liang Tian^a ; ¹ ; Fei He^b ; ¹ ; Xue Fu^a ; Jun-Tang Lin^c ; Ping Tang^a ; Yu-Min Huang^a ; Rong Guo^a ; ^{gh7311@aliyun.com" class="auth_mail} ; Ling Sun^a ; ^{lingsuncn@126.com" class="auth_mail}
• 关键词：Hsp90伪 ; heat shock protein 90 alpha ; Hsp90伪 ; Hsp90 alpha ; Hsp90尾 ; Hsp90 beta ; AL ; acute leukemia ; ALL ; acute lymphoblastic leukemia ; AML ; acute myeloid leukemia ; ELISA ; enzyme-linked immunosorbent assay ; BMMCs ; bone marrow mononuclear cells ; GAPDH ; glyceraldehyde 3-phosphate dehydrogenase ; CR ; complete remission ; NR ; non-remission ; GVHD ; graft versus host disease
• 刊名：Gene
• 出版年：1 June, 2014
• 年：2014
• 卷：542
• 期：2
• 页码：122-128
• 全文大小：778 K

文摘

This study investigated the expression of heat shock protein 90 alpha (Hsp90伪) in acute leukemia cells. The expression of Hsp90伪 was investigated in leukemia cell lines and human bone marrow mononuclear cells derived from acute leukemia patients and from healthy individuals using polymerase chain reaction, Western blot, and enzyme-linked immunosorbent assay. Compared with cells from healthy individuals, the expression of Hsp90伪 in the untreated patients was higher. Similarly high levels were observed in remission patients. Significantly higher expression levels were observed in all the tested cell lines, and in cells from refractory and relapsed patients. No obvious relationship was observed between the occurrence of graft versus host disease and the expression of Hsp90伪. The untreated patients showing higher expression levels of Hsp90伪 had lower complete remission rates. During remission of untreated patients, the expression of Hsp90伪 decreased and reached the lowest level after transplantation, but the expression increased again before relapse. Hsp90伪 was highly expressed in leukemia cells. The expression level of Hsp90伪 was associated with leukemia prognosis. However, no obvious relationship was observed between the occurrence of graft versus host disease and the expression of Hsp90伪.