Pleurotus nebrodensis polysaccharide induces apoptosis in human non-small cell lung cancer A549 cells

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• 作者：Haiyan Cui ; Changlu Wang ; ^{clw123@tust.edu.cn" class="auth_mail} ; ^{haiyancui2010@163.com" class="auth_mail} ; Yurong Wang ; Zhenjing Li ; Yanan Zhang ; Mianhua Chen ; Fengjuan Li
• 关键词：Pleurotus nebrodensis polysaccharides ; A549 cells ; Anticancer ; Apoptosis
• 刊名：Carbohydrate Polymers
• 出版年：15 April, 2014
• 年：2014
• 卷：104
• 期：Complete
• 页码：246-252
• 全文大小：1756 K

文摘

Polysaccharides derived from edible fungi inhibit the proliferation of tumor cells. In this study, we investigated the effects of a polysaccharide (PN50G) from Pleurotus nebrodensis on A549 cell proliferation and apoptosis. MTT assay showed that PN50G induced apoptosis in the A549 cells in a dose-dependent. However, PN50G did not affect the proliferation viability of human fetal lung fibroblast cells MRC-5. Scanning electro microscopy (SEM) results indicate that PN50G induced a typical apoptotic morphological feature in A549. DNA accumulation and fragmentation were determined by acridine orange/ethidium bromide (AO/EB) staining. Flow cytometric analysis demonstrated that PN50G caused A549 cell apoptosis via cell arrest at the S phase. PN50G also extended the comet tail length in single-cell gel electrophoresis and disrupted the mitochondrial membrane potential as determined by Rdamine-123 staining. Further analysis by qRT-PCR showed that the expression of caspase-3 and caspase-9 mRNA increased. These findings suggest that PN50G can inhibit A549 cell proliferation and induce apoptosis mainly by activating the intrinsic mitochondrial pathway.