Anti-inflammatory, analgesic and anti-tumor properties of gold nanoparticles

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• 作者：Raimundo Fernandes de Ara&uacute ; jo J&uacute ; nior^a ; ^b ; ^c ; ^{araujojr@cb.ufrn.br} ; Aurigena Antunes de Ara&uacute ; jo^d ; Jonas Bispo Pessoa^b ; Franscisco Paulo Freire Neto^e ; Gisele Ribeiro da Silva^d ; Ana Luiza C.S. Leitã ; o Oliveira^c ; Thaí ; s Gomes de Carvalho^c ; Heloiza F.O. Silva^f ; Mateus Eugê ; nio^g ; Celso Sant&rsquo ; Anna^g ; Luiz H.S. Gasparotto^f ; ^{lhgasparotto@ufrnet.br}
• 关键词：Gold nanoparticles ; Cancer cell apoptosis ; Anti-inflammatory activity ; Peripheral analgesia
• 刊名：Pharmacological Reports
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：69
• 期：1
• 页码：119-129
• 全文大小：7542 K
• 卷排序：69

文摘

Gold nanoparticles (GNPs) are regarded as potential platforms for drug delivery. However, their interaction with live organisms must be understood prior to their utilization as drug carriers. The present study reports the anti-inflammatory, analgesic and anti-tumor effects of GNPs. The biodistribution of GNPs and their effect on various tissues have also been studied.MethodsGNPs were synthesized through an environmentally friendly route and characterized with TEM and UV–vis. After HT-29 cells had been exposed to GNPs, apoptosis was assessed with Annexin V and propidium iodide staining and caspase-3 activity determined with a confocal laser scanning microscope. GNPs were administrated to male and female Swiss mice for posterior assessment of their anti-inflammatory and analgesic properties. The biodistribution of GNPs and their impact on tissues were studied with UV–vis and histopathological analysis, respectively.ResultsCell apoptosis was observed in a dose-dependent manner for GNPs concentrations ranging from 40 μg/mL to 80 μg/mL (p < 0.05). The best anti-inflammatory activity was observed at the dose of 1500 μg/kg, which caused a reduction of 49.3% in leukocyte migration. GNPs showed peripheral analgesia at the dose of 1500 μg/kg and have been found in liver, spleen, kidney and lungs. Histopathological examination revealed extravasation of red blood cells in lungs.ConclusionThe study draws attention to gold nanoparticles as a resource for technological innovation in the anti-inflammatory, analgesic and anti-tumor fields. GNPs have biological effects that deserve investigation to assess their full interaction with organic systems.