Chemiluminescence detection of lead (II) using 鈥?-17鈥?DNAzyme and hemin/G-quadruplex with high sensitivity and selectivity

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• 作者：Zi-Ming ZhouAuthor Vitae ; Yong YuAuthor Vitae ; Ming-Zhen ZhangAuthor Vitae ; Jun ChenAuthor Vitae ; Qiong-Qiong RenAuthor Vitae ; Ji-Tao SongAuthor Vitae ; Bo LiuAuthor Vitae ; Zhi-Ya MaAuthor Vitae ; Yuan-Di Zhao ; ^{zydi@mail.hust.edu.cn" class="auth_mail}Author Vitae
• 关键词：Chemiluminescence ; &lsquo ; 8&ndash ; 17&rsquo ; DNAzyme ; Lead ; Hemin/G-quadruplex
• 刊名：Sensors and Actuators B: Chemical
• 出版年：1 October 2014
• 年：2014
• 卷：201
• 期：Complete
• 页码：496-502
• 全文大小：1343 K

文摘

It is important to design new ways with high specificity and sensitivity to detect lead (II). Here, ‘8–17’ DNAzyme was used as a Pb²⁺-dependent probe with high sensibility and selectivity based on chemiluminescence (CL). When Pb²⁺ was present, the substrate strand was cleaved and the active hemin/G-quadruplex DNAzyme structure was formed in the presence of another sequence which contained two parts, one could be complementary with part of the substrate strand and the other was a G rich subunit. The active hemin/G-quadruplex DNAzyme structure could catalyze luminol oxidation and quantitative analysis of Pb²⁺ was carried out according to the CL of luminol. Results showed that CL intensity of luminol at 452 nm had a linear relation with the concentration of Pb²⁺ in the range from 5 nM to 1 渭M, and the limit of detection was 0.79 nM (S/N = 3). This method was also used to detect the contents of Pb²⁺ in lake water and tobacco and the results (62.4 ± 4.0 nM and 15.2 ± 2.2 nM, respectively) were extremely closed to those of inductively coupled plasma-mass spectrometry (ICP-MS) (57.0 ± 1.4 nM and 14.5 ± 0.91 nM, respectively).