Controlling joint instability delays the degeneration of articular cartilage in a rat model

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• 作者：K. Murata^&dagger ; ; ^&Dagger ; ; N. Kanemura^&Dagger ; ; ^{kanemura-naohiko@spu.ac.jp} ; T. Kokubun^&Dagger ; ; T. Fujino^&dagger ; ; Y. Morishita^&dagger ; ; K. Onitsuka^&dagger ; ; S. Fujiwara^&dagger ; ; A. Nakajima^&dagger ; ; D. Shimizu^&Dagger ; ; K. Takayanagi^&Dagger ;
• 关键词：Osteoarthritis ; Articular cartilage ; Joint instability ; Tumor necrosis factor alpha ; Caspase-3
• 刊名：Osteoarthritis and Cartilage
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：25
• 期：2
• 页码：297-308
• 全文大小：3219 K
• 卷排序：25

文摘

Joint instability induced by anterior cruciate ligament (ACL) transection is commonly considered as a predisposing factor for osteoarthritis (OA) of the knee; however, the influence of re-stabilization on the protection of articular cartilage is unclear. The aim of this study was to evaluate the effect of joint re-stabilization on articular cartilage using an instability and re-stabilization ACL transection model.DesignTo induce different models of joint instability, our laboratory created a controlled abnormal joint movement (CAJM) group and an anterior cruciate ligament transection group (ACL-T). Seventy-five Wistar male rats were randomly assigned to the CAJM (n = 30), ACL-T (n = 30), or no treatment (INTACT) group (n = 15). Cartilage changes were assessed with soft X-ray analysis, histological and immunohistochemistry analysis, and real-time polymerase chain reaction (PCR) analysis at 2, 4, and 12 weeks.ResultsJoint instability, as indicated by the difference in anterior displacement between the CAJM and ACL-T groups (P < 0.001), and cartilage degeneration, as evaluated according to the Osteoarthritis Research Society International (OARSI) score, were significantly higher in the ACL-T group than the CAJM group at 12 weeks (P < 0.001). Moreover, joint re-stabilization maintained cartilage structure (thickness [P < 0.001], surface roughness [P < 0.001], and glycosaminoglycan stainability [P < 0.001]) and suppressed tumor necrosis factor-alpha (TNF-α) and caspase-3 at 4 weeks after surgery.ConclusionRe-stabilization of joint instability may suppress inflammatory cytokines, thereby delaying the progression of OA. Joint instability is a substantial contributor to cartilage degeneration.