Curcumin attenuates blood-brain barrier disruption after subarachnoid hemorrhage in mice

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• 作者：Jichao Yuan ; MD¹ ; Wei Liu ; MD¹ ; Haitao Zhu ; MD ; Xuan Zhang ; MD ; Yang Feng ; MD ; Yaxing Chen ; MD ; Hua Feng ; MD ; Jiangkai Lin ; MD ; ^{jklin@tmmu.edu.cn}
• 关键词：Blood-brain barrier ; Curcumin ; Early brain injury ; Subarachnoid hemorrhage
• 刊名：Journal of Surgical Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：207
• 期：Complete
• 页码：85-91
• 全文大小：1219 K
• 卷排序：207

文摘

Early brain injury, one of the most important mechanisms underlying subarachnoid hemorrhage (SAH), comprises edema formation and blood-brain barrier (BBB) disruption. Curcumin, an active extract from the rhizomes of Curcuma longa, alleviates neuroinflammation by as yet unknown neuroprotective mechanisms. In this study, we examined whether curcumin treatment ameliorates SAH-induced brain edema and BBB permeability changes, as well as the mechanisms underlying this phenomenon.MethodsWe induced SAH in mice via endovascular perforation, administered curcumin 15 min after surgery and evaluated neurologic scores, brain water content, Evans blue extravasation, Western blot assay results, and immunohistochemical analysis results 24 h after surgery.ResultsCurcumin significantly improved neurologic scores and reduced brain water content in treated mice compared with SAH mice. Furthermore, curcumin decreased Evans blue extravasation, matrix metallopeptidase-9 expression, and the number of Iba-1-positive microglia in treated mice compared with SAH mice. At last, curcumin treatment increased the expression of the tight junction proteins zonula occludens-1 and occludin in treated mice compared with vehicle-treated and sample SAH mice.ConclusionsWe demonstrated that curcumin inhibits microglial activation and matrix metallopeptidase-9 expression, thereby reducing brain edema and attenuating post-SAH BBB disruption in mice.