MicroRNA and protein profiles in invasive versus non-invasive oral tongue squamous cell carcinoma cells in vitro

详细信息    查看全文

• 作者：Johanna Korvala^a ; ^{johanna.korvala@oulu.fi} ; Kowan Jee^b ; ^c ; Emmi Porkola^a ; Alhadi Almangush^c ; Neda Mosakhani^d ; Carolina Bitu^a ; Nilva K. Cervigne^e ; ^f ; Flá ; via S. Zandonadi^g ; Gabriela V. Meirelles^g ; Adriana Franco Paes Leme^g ; Ricardo D. Coletta^e ; Ilmo Leivo^b ; ^c ; Tuula Salo^a ; ^h
• 关键词：DMEM ; Dulbecco&rsquo ; s Modified Eagle&rsquo ; s medium ; ECM ; extracellular matrix ; FBS ; fetal bovine serum ; HSC-3 ; human squamous cell carcinoma cell line ; IIS ; Integrated interactome system ; miRNA ; microRNA ; SCC-15 ; human squamous cell carcinoma cell line ; qRT-PCR ; quantitative reverse transcription-PCR
• 刊名：Experimental Cell Research
• 出版年：2017
• 出版时间：1 January 2017
• 年：2017
• 卷：350
• 期：1
• 页码：9-18
• 全文大小：1467 K
• 卷排序：350

文摘

Complex molecular pathways regulate cancer invasion. This study overviewed proteins and microRNAs (miRNAs) involved in oral tongue squamous cell carcinoma (OTSCC) invasion. The human highly aggressive OTSCC cell line HSC-3 was examined in a 3D organotypic human leiomyoma model. Non-invasive and invasive cells were laser-captured and protein expression was analyzed using mass spectrometry-based proteomics and miRNA expression by microarray. In functional studies the 3D invasion assay was replicated after silencing candidate miRNAs, miR-498 and miR-940, in invasive OTSCC cell lines (HSC-3 and SCC-15). Cell migration, proliferation and viability were also studied in the silenced cells. In HSC-3 cells, 67 proteins and 53 miRNAs showed significant fold-changes between non-invasive vs. invasive cells. Pathway enrichment analyses allocated “Focal adhesion” and “ECM-receptor interaction” as most important for invasion. Significantly, in HSC-3 cells, miR-498 silencing decreased the invasion area and miR-940 silencing reduced invasion area and depth. Viability, proliferation and migration weren’t significantly affected. In SCC-15 cells, down-regulation of miR-498 significantly reduced invasion and migration. This study shows HSC-3 specific miRNA and protein expression in invasion, and suggests that miR-498 and miR-940 affect invasion in vitro, the process being more influenced by mir-940 silencing in aggressive HSC-3 cells than in the less invasive SCC-15.