Increased intracellular Ca²⁺ decreases cisplatin resistance by regulating iNOS expression in human ovarian cancer cells

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• 作者：Yang Yu^a ; ¹ ; Qi Xie^b ; ¹ ; Weimin Liu^c ; Yuting Guo^a ; Na Xu^a ; Lu Xu^a ; Shibing Liu^a ; Songyan Li^a ; Ye Xu^a ; ^{xuye_9707@163.com} ; Liankun Sun^b ; ^{sunlk@jlu.edu.cn}
• 关键词：Ovarian cancer ; Cisplatin resistance ; Intracellular Ca2+ ; iNOS
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：86
• 期：Complete
• 页码：8-15
• 全文大小：3162 K
• 卷排序：86

文摘

Previous studies have reported that intracellular Ca2+ signals and inducible nitric oxide synthase (iNOS) are involved in cell apoptosis. However, the role of iNOS in cisplatin resistance in ovarian cancer remains unclear. Here, we demonstrate that SKOV3/DDP ovarian cancer cells were more resistant to cisplatin than were SKOV3 ovarian cancer cells. The expression of intracellular Ca2+ and iNOS was more strongly induced by cisplatin in SKOV3 cells than in SKOV3/DDP cells. TAT-conjugated IP3R-derived peptide (TAT-IDPS) increased cisplatin-induced iNOS expression and apoptosis in SKOV3/DDP cells. 2-Aminoethoxydiphenyl borate (2-APB) decreased cisplatin-induced iNOS expression and apoptosis in SKOV3 cells. Thus, iNOS induction may be a valuable strategy for improving the anti-tumor efficacy of cisplatin in ovarian cancer.