The hydrophilic and negatively charged PU-CS films were successfully prepared by chemical grafting using N-Boc-1,3-propanediamine as a spacer and the chondroitin sulfate (CS) grafting density on PU films can be controlled by the mass fraction of the spacer. The impacts of the CS grafting density on the adsorption of proteins and adhesion of glycosaminoglycans were evaluated. The impact of inorganic salt deposition after conditioning film formation (co-adsorption of proteins and polysaccharides) on PU and PU-CS(3) film was investigated with artificial urine samples.