Establishment of 3D culture and induction of osteogenic differentiation of pre-osteoblasts using wet-collected aligned scaffolds

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• 作者：Huifen Ding^a ; ^b ; Junwen Zhong^c ; Fei Xu^a ; Fangfang Song^a ; Miao Yin^a ; Yanru Wu^a ; Qiyi Hu^a ; Jiawei Wang^a ; ^{wangjwei@hotmail.com}
• 关键词：ECM ; extracellular matrix ; SF ; silk fibroin ; AD ; aligned scaffolds collected by dry process ; RD ; random scaffolds collected with dry process ; AW ; aligned scaffolds collected by wet process ; RW ; random scaffolds collected with wet process ; SEM ; scanning electron microscopy ; PBS ; phosphate buffer solution ; FTIR ; Fourier transform infrared spectrum ; ALP ; alkaline phosphatase
• 刊名：Materials Science and Engineering: C
• 出版年：2017
• 出版时间：1 February 2017
• 年：2017
• 卷：71
• 期：Complete
• 页码：222-230
• 全文大小：2264 K
• 卷排序：71

文摘

Aligned silk fibroin nanofibres were loosely packed using a novel wet-collection electrospinning method. Structural properties of the aligned nanofibres were improved. Three-dimensional culture of MC3T3-E1 pre-osteoblasts was established. The arrangement, morphology, infiltration, proliferation and osteogenic differentiation of cells were enhanced.