Enhanced legumain-recognition and NIR controlled released of cisplatin-indocyanine nanosphere against gastric carcinoma

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• 作者：Tianyi Shi^a ; Lianshuai Gu^a ; Yu Sun^a ; Senlin Wang^a ; Chaoqun You^a ; Xiangyang Zhang^b ; Jin Zhu^c ; Baiwang Sun^a ; ^{chmsunbw@seu.edu.cn}
• 关键词：Lipid-polymer nanoparticle ; Legumain-recognition ; Controlled-release ; Drug delivery ; Cisplatin ; Indocyanine
• 刊名：European Journal of Pharmacology
• 出版年：2017
• 出版时间：5 January 2017
• 年：2017
• 卷：794
• 期：Complete
• 页码：184-192
• 全文大小：1672 K
• 卷排序：794

文摘

Cisplatin-therapy has faced limitations in the gastric cancer therapy. To settle the bottleneck, enhanced specificity and controlled-release property are choosen.We synthesize cisplatin and indocyanine green (ICG) loaded PLGA-(DSPE-PEG2000) nanoparticles, which is abbreviated as CINPs. And we conjugate the Gly-Cys-Gly-Ala-Ala-Asn-Leu (GCGAANL) heptapeptide upon the surface of CINPs, the product is abbreviated as ACINPs. ACINPs with nearly 110 nm exhibit good monodispersity and size stability. The EE (efficiency of encapsulation) and LE (loading of encapsulation) of cisplatin loaded into ACINPs are optimized as 29.81% and 3.88%.MGC803 cells overexpressing the legumain and MKN28 cells, which negatively express the legumain as well as the normal stomach cells, are selected. In vitro studies have suggested ACINPs, compared with CINPs, could be recognized by MGC803 cells and efficiently killed the cancer cells, while be harmless to MKN28 cells, which indicates the specificity and safety of ACINPs.Under irradiation of 808 nm NIR irradiation, ICG loaded in ACINPs could rapidly transform the light to heat up to 60 ℃. Nanoparticles compared with non-irraditaion group could be quickly disrupted and release the cisplatin which could enhance the controlled-release ability.Hence, the ACINPs exhibit great potential in avoiding the side effects and enhancing the therapy ability of cisplatin.