AAV-mediated transfer of RhoA shRNA and CNTF promotes retinal ganglion cell survival and axon regeneration

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• 作者：Ling-Ping Cen^a ; ^{cenlp@hotmail.com} ; Jia-Jian Liang^a ; Jian-huan Chen^a ; Alan R. Harvey^c ; Tsz Kin Ng^b ; Mingzhi Zhang^a ; Chi Pui Pang^a ; ^b ; Qi Cui^a ; ^b ; You-Ming Fan^a ; ^d ; ^{youmingf@aliyun.com}
• 关键词：AAV ; adeno-associated viral vector ; ANOVA ; analysis of variance ; BDNF ; brain-derived neurotrophic factor ; CNTF ; ciliary neurotrophic factor ; CNTF ; ciliary neurotrophic factor ; CSPGs ; chondroitin sulfate proteoglycans ; GCL ; ganglion cell layer ; ON ; optic nerve ; ONC ; optic nerve crush ; RGCs ; retinal ganglion cells
• 刊名：Neuroscience
• 出版年：2017
• 出版时间：20 February 2017
• 年：2017
• 卷：343
• 期：Complete
• 页码：472-482
• 全文大小：2592 K
• 卷排序：343

文摘

AAV-RhoA shRNA decreased RhoA expression and promoted neurite outgrowth in vitro. AAV-CNTF promoted RGC survival and axonal regeneration in vivo. AAV-RhoA shRNA alone has weak effects on axonal regeneration in vivo. AAV-RhoA shRNA improved therapeutic effect of AAV-CNTF by two fold in combination.