An enzymatic assay based on luciferase Ebola virus-like particles for evaluation of virolytic activity of antimicrobial peptides

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• 作者：Marie Peskova^a ; ^{m.peskova@mail.muni.cz} ; Zbynek Heger^b ; ^c ; ^{zbynek.heger@mendelu.cz} ; Petr Janda^a ; ^{323853@mail.muni.cz} ; Vojtech Adam^b ; ^c ; ^{vojtech.adam@mendelu.cz} ; Vladimir Pekarik^a ; ^d ; ^{pekarikv@mail.muni.cz}
• 关键词：Virus-like particles ; Cytotoxic peptides ; Antimicrobial peptides ; Nanoluciferase ; Hemolytic activity ; Virolytic activity ; Lentivirus ; Ebola ; Marburg
• 刊名：Peptides
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：88
• 期：Complete
• 页码：87-96
• 全文大小：2535 K
• 卷排序：88

文摘

Virolytic assay for pore forming peptides based on Ebola virus-like particles. Applicability to search for Ebola and Marburg virus inactivating compounds. Systematic comparison of pore forming peptides in hemolytic and virolytic assays. Cecropin A/Melittin hybrid (CAM-W) destroys infectivity of lentiviruses. CAM-W effectively lyses Ebola virus-like particles at micromolar concentrations.