Improved formulation of cationic solid lipid nanoparticles displays cellular uptake and biological activity of nucleic acids

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• 作者：Anna Fà ; bregas^a ; ¹ ; Silvia Prieto-Sá ; nchez^b ; ¹ ; Marc Su&ntilde ; é ; -Pou^a ; ^b ; ¹ ; Sofí ; a Boyero-Corral^b ; Josep Ramó ; n Ticó ; ^a ; ^c ; Encarna Garcí ; a-Montoya^a ; ^c ; Pilar Pé ; rez-Lozano^a ; ^c ; Montserrat Mi&ntilde ; arro^a ; ^c ; Josep Mª ; Su&ntilde ; é ; -Negre^a ; ^c ; Cristina Herná ; ndez-Munain^d ; Carlos Su&ntilde ; é ; ^b ; ^{csune@ipb.csic.es}
• 关键词：Stearic acid (PubChem CID ; 5281) ; Octadecylamine (PubChem ID ; 15793) ; Poloxamer 188 (PubChem CID ; 24751)
• 刊名：International Journal of Pharmaceutics
• 出版年：2017
• 出版时间：10 January 2017
• 年：2017
• 卷：516
• 期：1-2
• 页码：39-44
• 全文大小：1049 K
• 卷排序：516

文摘

Non-viral delivery using cationic solid lipid nanoparticles (SLNs) represents a useful strategy to introduce large DNA and RNA molecules to target cells. A careful selection of components and their amounts is critical to improve transfection efficiency. In this work, a selected and optimized formulation of SLNs was used to efficiently transfect circular DNA and linear RNA molecules into cells. We characterized the main physicochemical characteristics and binding capabilities of these SLNs and show that they deliver DNA and RNA molecules into cells where they display full bioactivity at nontoxic concentrations using fluorescence- and luminescence-based methodologies. Hence, we established a novel and simple SLN formulation as a powerful tool for future therapeutic use.