Chenopodium album Linn. leaves prevent ethylene glycol-induced urolithiasis in rats

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• 作者：Indu Sikarwar^a ; Yadu Nandan Dey^b ; Manish M. Wanjari^b ; ^{manish.nriashrd@gmail.com} ; Ajay Sharma^a ; Sudesh N. Gaidhani^c ; Ankush D. Jadhav^b
• 关键词：Bathua ; Urolithiasis ; Ethylene glycol ; Cystone ; Calcium oxalate
• 刊名：Journal of Ethnopharmacology
• 出版年：2017
• 出版时间：4 January 2017
• 年：2017
• 卷：195
• 期：Complete
• 页码：275-282
• 全文大小：847 K
• 卷排序：195

文摘

The leaves of Chenopodium album Linn. are traditionally used for correction of kidney diseases and urinary stones. The present work investigated the effect of methanolic and aqueous extracts of leaves of Chenopodium album on experimentally-induced urolithiasis in rats to substantiate its traditional use as antilithiatic agent.Materials and methodsThe leaf extract was standardized by HPLC. Urolithiasis was induced in rats by administration of 0.75% v/v of ethylene glycol (EG) in distilled water and in addition, vehicle or methanol (CAME) or aqueous (CAAE) extract of the leaves of Chenopodium album each in the dose 100, 200 and 400 mg/kg or Cystone (750 mg/kg) were administered daily orally for 28 days. Urolithiasis was assessed by estimating the calcium, phosphorus, urea, uric acid, and creatinine in both urine and plasma. The volume, pH and oxalate levels were also estimated in urine. The renal oxalate content was estimated in kidney while calcium oxalate deposits were observed histologically.ResultsThe treatment with CAME or CAAE for 28 days significantly attenuated the EG-induced elevations in the urine and plasma levels of calcium, phosphorus, urea, uric acid and creatinine along with decrease in urine volume, pH and oxalates. The treatments also decreased renal tissue oxalate and deposition of oxalate crystals in kidney due to EG treatment. The effects of CAME and CAAE were comparable to standard antilithiatic agent, cystone. The findings indicate the preventive effect of CAME and CAAE which can be due to inhibitory effect on crystallization and stone dissolution. The effect was attributed to the presence of phytochemicals like flavonoids and saponins.ConclusionIn conclusion, Chenopodium album leaves exhibited antilithiatic effect and validates its ethnomedicinal use in urinary disorders and kidney stones.