Repeated whole cigarette smoke exposure alters cell differentiation and augments secretion of inflammatory mediators in air-liquid interface three-dimensional co-culture model of human bronchial tissue

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• 作者：Shinkichi Ishikawa ; ^{shinkichi.ishikawa@jt.com} ; Shigeaki Ito
• 关键词：3D ; three-dimensional ; AEGM ; Airway Epithelial Cell Growth Medium ; ALI ; air-liquid interface ; CS ; cigarette smoke ; CSE ; cigarette smoke extract ; COPD ; chronic obstructive pulmonary disease ; CYP ; cytochrome P450 ; FBS ; fetal bovine serum ; HBECs ; human bronchial epithelial cells ; ISO ; International Organization for Standardization ; LDH ; lactate dehydrogenase ; MEM ; minimum essential medium ; OECD ; Organization for Economic Co-operation and Development
• 刊名：Toxicology in Vitro
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：38
• 期：Complete
• 页码：170-178
• 全文大小：1364 K
• 卷排序：38

文摘

Human bronchial tissue was exposed to whole cigarette smoke in an air-liquid interface three-dimensional co-culture model. Repeated whole cigarette smoke exposure decreased ciliated cell differentiation and altered goblet cell differentiation. Repeated whole cigarette smoke exposure induced secretion of several mediators (IL-8, GRO-α, IL-1β, GM-CSF). Secretion of these mediators was augmented with repetition of the exposure.