Analgesic effect of coumarins from Radix angelicae pubescentis is mediated by inflammatory factors and TRPV1 in a spared nerve injury model of neuropathic pain

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• 作者：Ruili Li^a ; ¹ ; Chao Zhao^a ; ¹ ; Minna Yao^a ; Ying Song^b ; Yin Wu^a ; ; Aidong Wen^a ; ^{lwrcll@163.com}
• 关键词：CRAP ; Coumarins from Radix angelicae pubescentis ; SNI ; spared nerve injury ; DRG ; dorsal root ganglion ; CRAP ; coumarins from Radix angelicae pubescentis ; IL-1β ; interleukin-1β ; IL-6 ; interleukin-6 ; TNF-α ; tumor necrosis factor-α ; TRPV1 ; Transient receptor potential cation channel 1 ; pERK ; Phosphorylated extracellular regulated protein kinases ; PKC ; protein kinase C ; MAPKs ; mitogen-activated protein kinases ; LOD ; limit of detection ; LOQ ; limit of quantification ; ELISA ; Enzyme-linked immunosorbent
• 刊名：Journal of Ethnopharmacology
• 出版年：2017
• 出版时间：4 January 2017
• 年：2017
• 卷：195
• 期：Complete
• 页码：81-88
• 全文大小：810 K
• 卷排序：195

文摘

Coumarins from Radix angelicae pubescentis (CRAP) are a major active component that are isolated from dried roots of Angelica biserrata Yuan et Shan, which has been used clinically to cure headaches for a long period of time, and it is an effective treatment for pain. The aim of the present study was to investigate the analgesic effect of CRAP on a spared nerve injury (SNI) model of neuropathy.Materials and methodsAntinociceptive effects of CRAP were assessed in Sprague-Dawley male rats using a spared nerve injury model of neuropathic pain. Inflammatory factors were determined by Enzyme-linked immunosorbent assay (ELISA). Transient receptor potential cation channel 1 (TRPV1) and Phosphorylated extracellular regulated protein kinases (pERK) were detected by Immunofluorescence and Western blotting, respectively.ResultsThe high performance liquid chromatography (HPLC) analysis showed the presence of osthole and columbianadin in Radix angelicae pubescentis. CRAP induced the dose-dependent effect of on attenuating the development of mechanical hypersensitivity. Molecular profiling revealed that CRAP reduced the levels of pro-inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) and significantly attenuated the expression of TRPV1 and pERK in damaged DRG neurons.ConclusionThis results demonstrate that CRAP possess remarkable antinociceptive activities which may be due to osthole and columbianadin at least in part, supporting the folkloric usage of the plant to treat various pain diseases.