Critical role of tumor necrosis factor-α in the early process of wound healing in skin

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• 作者：Masae Ritsu^a ; Kazuyoshi Kawakami^b ; Emi Kanno^c ; Hiromasa Tanno^c ; Keiko Ishii^b ; Yoshimichi Imai^a ; Ryoko Maruyama^c ; Masahiro Tachi^a ; ^{tachi@med.tohoku.ac.jp}
• 关键词：TNF-α ; tumor necrosis factor-α ; mAb ; monoclonal antibodies ; ELISA ; enzyme-linked immunosorbent assay ; HE ; hematoxylin-eosin ; SD ; standard deviation
• 刊名：Journal of Dermatology & Dermatologic Surgery
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：21
• 期：1
• 页码：14-19
• 全文大小：1194 K
• 卷排序：21

文摘

Tumor necrosis factor (TNF)-α is quickly released and initiates inflammation at wound tissues, but its precise role in wound healing is not fully understood. We examined the contribution of this cytokine to the early process of healing using a mouse model with full-thickness wounds in skin. TNF-α synthesis was detected just after wound creation, increased during the first several hours, reached a peak level on day 1, and then decreased to the basal level. In mice treated with anti-TNF-α mAb, wound closure was significantly delayed, and distances between the panniculus carnosus edges were significantly longer on day 3, compared with control. Inflammatory cell and fibroblast density were markedly decreased on day 3 in the anti-TNF-α mAb-treated mice compared with control. In contrast, wound healing was accelerated on day 3 when mice were treated with bioactive TNF-α. These results indicate that TNF-α is involved in the early process of wound healing.