Determination of ginsenosides in Asian and American ginsengs by liquid chromatography-quadrupole/time-of-flight MS: assessing variations based on morphological characteristics

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• 作者：Yujie Chen¹ ; ² ; ³ ; Zhongzhen Zhao¹ ; ⁴ ; Hubiao Chen¹ ; ⁴ ; Eric Brand¹ ; Tao Yi¹ ; Minjian Qin³ ; ^{minjianqin@163.com} ; Zhitao Liang¹ ; ⁴ ; ^{lzt23@hkbu.edu.hk}
• 关键词：determination of ginsenosides ; morphological characteristics ; Panax ginseng ; Panax quinquefolius ; ultra-HPLC&ndash ; quadrupole/time-of-flight MS
• 刊名：Journal of Ginseng Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：41
• 期：1
• 页码：10-22
• 全文大小：1811 K
• 卷排序：41

文摘

Asian ginseng and American ginseng are functional foods that share a close genetic relationship and are well-known worldwide. This article aims to investigate the correlation between morphological characteristics and the inherent quality of Asian and American ginsengs.MethodsIn this study, an ultra-HPLC–quadrupole/time-of-flight MS (UHPLC-Q/TOF-MS) method was established for the quantitative analysis of 45 ginseng samples. The method developed for determination was precise and accurate.ResultsThe results showed that Asian ginseng samples with the same growing time (with the same or similar number of stem scars) that had a thinner main root, a longer rhizome and more branch roots contained greater amounts of ginsenosides. For American ginseng, two tendencies were observed in the relationship between the diameter of the main root and contents of ginsenosides. One tendency was that samples with thinner main roots tended to contain higher levels of ginsenosides, which was observed in the samples sold under the commercial name pao-shen. Another tendency was that samples with thicker main roots contained higher contents of ginsenosides, which was observed in the samples sold under the commercial name pao-mian, as well as in samples of American ginseng cultivated in Jilin, China.ConclusionAn approach using ultra-HPLC–quadrupole/time-of-flight MS was successfully established to link morphology and active components for evaluating the quality of Asian and American ginsengs. Clear correlation between visible morphological features and quality of Asian and American ginsengs was found. People can see the difference; this means consumers and vendors can evaluate ginseng by themselves.